中文名稱:人B細胞活化因子受體(BAFF-R)ELISA試劑盒
英文名稱:The human B- cell activating factor receptor (BAFF-R) ELISA Kit
規 格:96T/Kit (8*12 strips) 48T/Kit (8*6 strips)
保存和有效期:2-8℃/6個月
運輸條件:低溫運輸,用干冰或者冰袋低溫運輸。
可測種屬:人、大小鼠、豚鼠、兔子、豬、犬、猴、馬、、、、、、
可測標本:血清、血漿、細胞上清液、尿液、體液、灌洗液、、、、、、、
定義:一種酶聯免疫技術,用于檢測被于固相板孔中的待測抗原(或抗體)。即用酶標記抗體,并將已知抗原或抗體吸附在固相載體表面,使抗原抗體反應在固相載體表面進行,用洗滌法將液相中的游離成分洗除,zui后通過酶作用于底物后顯色來判斷結果。
人B細胞活化因子受體(BAFF-R)ELISA試劑盒專一性強、靈敏度高、樣品易保存。
應用學科:免疫學(一級學科);應用免疫(二級學科);免疫學檢測和診斷(二級學科)
人B細胞活化因子受體(BAFF-R)ELISA試劑盒必須遵守以下3個核心原理:
1)抗原或抗體能吸附于固相載體表面,并保持其免疫學活性;
2)抗原或抗體可通過共價鍵與酶連接形成酶結合物,而此種酶結合物仍能保持其免疫學和酶學活性;
3)酶結合物與相應抗原或抗體結合后,可根據加入底物的顏色反應來判定是否有免疫反應的存在,而且顏色反應的深淺是與標本中相應抗原或抗體的量成正比例的,可根據已知濃度的抗原或抗體的顏色反應的深淺繪制標準曲線并依此計算出未知樣品中抗體或抗原的濃度。在ELISA中酶起到關鍵的作用,常用于ELISA法的酶有辣根過氧化物酶,堿性磷酸酯酶等,其中以辣根過氧化物酶為多,人B細胞活化因子受體(BAFF-R)ELISA試劑盒酶結合物是酶與抗體或抗原,半抗原在交聯劑作用下聯結的產物,它不僅具有抗體抗原特異的免疫反應,還能催化酶促反應,顯示出生物放大作用。
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