非洲綠猴腎細胞 BS-C-1產品運輸方式：干冰或者活體細胞運輸，本公司會保證細胞運輸中的存活率本公司細胞株低價保證質量，免費快遞直達。本公司擁有的專業細胞培養技術人員為您的細胞培養準備詳細的技術咨詢。齊一生物科技(上海）有限公司：。Web:www.qiyibio。。ｃｏｍ

非洲綠猴腎細胞 BS-C-1一、細胞操作參考書
二、生長特性：貼壁    懸浮
三、細胞生長條件：
培養基                  90%              （GIBCO）
血清              10%  FBS（GIBCO）
溫度                       37℃
空氣條件      5% CO2，,95% AIR
生長代數    P4
凍存條件    培養基70%、血清20%、DMSO10%
四、組成：
組份    規格
細胞一瓶    T25
細胞培養與操作說明    1份
五、細胞接收后的操作流程與注意事項
1.如果細胞為貼壁細胞，，而收到時呈懸浮或者部分懸浮的狀態，請將懸浮的細胞即時離心，加15%血清的*培養基到新的培養皿/瓶繼續培養3天；同時原培養瓶中剩下的貼壁細胞更換為15%血清的*培養基，培養3天。3天后若原瓶或者新瓶中的細胞都沒有出現增殖而是繼續脫落死亡，請及時實驗室，技術人員會跟進解決
2.貼壁細胞生長緩慢：適當提高血清濃度（zui高不能超過20%），或可根據該細胞生長密度，考慮*消化后，轉移到新的培養瓶繼續培養
3.生長不均：貼壁細胞若出現分布不均，成島狀生長，可將細胞進行消化，重懸打散細胞，加入新鮮培養基進行培養

六、細胞常規培養傳代流程（請嚴格遵照無菌操作）
1．吸出原培養瓶中的培養基，PBS緩沖液潤洗細胞兩次，加2~3 ml  0.25%*進行消化細胞（注意把握消化時間，通常控制在1-2min）
2．鏡下觀察消化情況，在細胞邊緣縮小，貼壁松動時（不建議消化到細胞漂浮）去掉*，加6~8ml *培養基，輕輕吹打細胞層，盡量把細胞層吹落，吹散
3．取部分細胞懸液轉移到新的培養皿/瓶中，添加適當的*培養基，，把細胞懸液打勻，于培養箱中培養
4．注意培養基PH值變化情況，定期換液（每周2-3次），待細胞密度達到80%以后重復1項操作或者凍存
特別注意：（如使用公共實驗室或初次接觸細胞培養，建議添加雙抗培養）
1.收到細胞后請盡快更換為含15%血清的新鮮培養基，如因特殊情況需要繼續使用原瓶培養基，請在原瓶培養基中額外添加10%的血清（原瓶培養基的繼續使用時間zui長不宜超過72小時）
2.貼壁細胞收到當天切忌立刻消化，請將細胞放置培養箱孵育過夜到第二天再做消化傳代
如簽收時出現培養瓶壁破裂，漏液等情況請及時做好照片記錄并實驗室
Instrument Part    9014967    Z-Module 1100μl, Soldered, BR8 MDx v1.0
Consumable    956034    EZ1 RNA Universal Tissue Kit (48)    Provides automated purification of total RNA from 1-6 samples of all types of animal or human tissue, including difficult-to-lyse fibrous and fatty tissues, or cultures cells.
Consumable    204645    QuantiTect Multiplex RT-PCR Kit (1000)
Consumable    37623    Qproteome FFPE Tissue Kit (20)
Consumable    158788    Gentra Puregene Cell Kit Plus (6.7x109)
Consumable    949016    Autopure 99% Isopropanol (3800 ml)
Instrument Part    981008    PCR Tubes, 0.2 ml  (10000)
Consumable    330421    RT2 Easy First Strand Kit (12)
Consumable    330612    RT2 SYBR Green Fluor FAST Mastermix (12)
Consumable    330521    RT2 SYBR Green ROX qPCR Mastermix (8)
Instrument Part    9020936    Kit, calibration, mech. variation, RGQ
Instrument Part    9020912    Lid, SMC, QGY
Instrument Part    9020939    Vacuum Pump, incl. trap and filters, QXT
Instrument Part    9020697    Fan, electrical, 80mm, C1x
Instrument Part    9020678    Rail, centre, CX1
    9023562    Kit, calibration, QXP
Instrument Part    9012945    Liquid Handling Kit Plato 3000 (500ul)
Instrument Part    9013550    Set, Fuses,  (220 + 110V) Plato 1/3
Instrument Part    9019336    Connector, JP5, Hi, Med, Lo, RG6
Instrument Part    9019350    Heater assembly, element, sensors, RG6
Instrument Part    9019397    Belt, toothed, rotor drive, RG3
Instrument Part    9019428    Sensor, source home, RG3
Instrument Part    9019443    Bridge rectifier 50A / 400V
    9019476    Spacer, 0.2 mm short set, GSC
    9019477    LASER MODUAL GS
Instrument Part    9019532    Tip ejector, QGY
Instrument Part    9019547    Fan guard and filter, Cxx
Instrument Part    9016732    Security Key, LC Policy Manager, encoded
Consumable    158267    Gentra Puregene Mouse Tail Kit (4 g)
Consumable    158916    DNA Hydration Solution (500 ml)
Consumable    949010    Autopure DNA Hydration Soln. (3800 ml)
Instrument Part    9018697    EZ1 Adv. XL DNA Buffy Coat Card
Instrument Part    9019813    Service kit, pipettor 8 ch w/tool, Cx8
Instrument Part    9019839    PyroMark Computer (OPC), PyroQ96
Consumable    204852    QuantiFast Multiplex RT-PCR Kit (80)
Consumable    336314    SureSilencing shRNA Plasmid, Puromycin
Consumable    338112    Human XpressRef Universal Total RNA
Consumable    336831    Cignal Finder Lenti Reporter Array
Consumable    338116    Rat XpressRef Universal Total RNA
Consumable    330510    RT2 SYBR Green Fluor qPCR Mastermix (2)
Consumable    4501363    artus EBV QS-RGQ Kit(24) CE
Instrument Part    9242548    Cheetah Upgrade Kit, AP98
Instrument Part    9020903    Gas strut, lid, 100 N, SMC, QGX
    9023554    Lamp, w filter, QXP
Instrument Part    9012744    Peristaltic Pump outlet tubing 1.25 m
Instrument Part    9015310    Unit, door lock, M48
Instrument Part    9015315    Belt, X-iming, M48
Instrument Part    9015320    Belt, Z-motor, M48
Consumable    74204    RNeasy MinElute Cleanup Kit (50)    50 RNeasy MinElute Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers
Consumable    207045    QuantiTect Whole Transcriptome Kit (100)
Instrument Part    9019348    Impeller, heater fan, RGx
Instrument Part    9019355    Rotor hub with shaft, RG6
Instrument Part    9019399    Damper, lid, RG3
Instrument Part    9019408    Heater assy  incl. element, sensors, RG3
Instrument Part    9019418    Kit, heater, FET, ICs, diode, cap, RG3
    9019469    COMB CASTING 0.2MM X 52 WELLS
Instrument Part    9019535    Motor, X-axis, Cxx
Instrument Part    9238475    2ml O-ring adapter tips, RapidPlate
Consumable    80204    AllPrep DNA/RNA Mini Kit (50)    50 Allprep DNA Spin Columns, 50 Rneasy Columns, Collection Tubes, Rnase Free Reagents and Buffers
Consumable    158445    Gentra Puregene Blood Kit (30 ml)
Consumable    158908    Cell Lysis Solution (1000 ml)
Consumable    949002    Autopure Glycogen Solution (5 ml)
Consumable    949006    Autopure Cell Lysis Solution (3800 ml)
Consumable    206403    QIAGEN LongRange PCR Kit (250)    For 250 PCR rxns a 50 μl: Enzyme Mix (Taq and Proofreading Enzyme, (5 u/μl), 10 x PCR Buffer for increased fidelity (contains dNTP′s),  25 mM MgCl2, 5 x Q-Solution
Consumable    216213    FastLane Cell SYBR Green Kit (200)
Consumable    12991    QIAGEN Plasmid Plus Giga Kit (5)    QIAGEN Plasmid Plus Kits enable ultrafast, large-scale plasmid purification of pure plasmid DNA. The use of a vacuum manifold allows purification of up to 12 (Mega and Giga) or up to 24 (Midi and Maxi) samples in parallel with low hands-on needs. Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. QIAGEN Plasmid Plus Kits also feature a novel wash buffer for endotoxin reduction.
Instrument Part    9018705    EZ1 Adv. XL RNA Card
Instrument Part    9019815    Service kit, 1 channel pipettor, CAS1200
Instrument Part    9019816    Gasket, cooling flap, RG6
Consumable    204952    QuantiFast Multiplex RT-PCR +R Kit (80)
Instrument Part    9019168    Set, O-Ring (32), Qsym
Consumable    330512    RT2 SYBR Green Fluor qPCR Mastermix (12)
Consumable    336313    SureSilencing shRNA Plasmid, Neomycin
Consumable    338174    RT2 Calibration Plate D
Consumable    330141    Custom RT2 PreAMP Primer Mix
Consumable    330619    RT2 SYBR Green Fluor FAST Mastermix 25ml
Instrument Part    9020901    Multi.Carrier (2) for 7 cult.tubes 50ml
Instrument Part    9020470    Cover, front, GSC
Instrument Part    9020904    Lid, SMC, QXT
Instrument Part    9020709    Cable, y, z, & p motor harness, C34
Instrument Part    9012750    Syringe pump outlet tubing (5.7 m)
Instrument Part    9015311    Unit, spotlight, left, M48
Instrument Part    9015323    O-rings, plunger fixing, M48 (6)
Consumable    953336    MagAttract DNA Mini M48 Kit (192)    For 192 DNA preps: MagAttract Suspension B, Buffers, Proteinase K
Instrument Part    9015094    Vacuum Manifold Top, SBS
Instrument Part    9019342    LED, IR, RG6
Instrument Part    9019344    Sensor, optics source home, RG6
Instrument Part    9019359    Filter, fuse, switch mains power, RGx
Instrument Part    9019366    Wiring, lower heater, RG6
Instrument Part    9019383    Board, main, standard, RG3
Instrument Part    9019395    Fan, 120mm, rear panel, RG3
Instrument Part    9019398    Solenoid, lid lock, RG3
Instrument Part    9019430    Hinge, front rear hinge plates, RG3
Instrument Part    9019442    Driver board V 5.1
Instrument Part    9019449    Mounting plate (IPAQ series) comp
Instrument Part    9019499    ReagBlock, 8x1.5ml/2ml/1.5ml Ta/Flat/Ta
    9019500    UV light system, CAS
Instrument Part    9019549    Pin, lid hinge, CAS
Instrument Part    9019567    Clamp Bracket, Y-arm mounting, Cxx
Instrument Part    9019570    Clamp, cable 3 wires 2 tabs thru, Cxx
Instrument Part    9020289    Tool, Adj., Extractor, Qsym
Instrument Part    9020253    Waste bag, Qsym
Instrument Part    9020602    Packing Foam Set - DML2000
Instrument Part    9020628    Door, consumables, right, Cabinet Qsym
Consumable    386015    DNA Size Standard 550 (BTO) (100)
    1061113    PG AIV-H7 RT-PCR Assay (48) Z
    4874004    PG AIV-H7 RT-PCR Kit (48)  Z
Instrument Part    9022323    Power Supply, Luminometer, careHPV
Consumable    331260    miScript PreAMP Custom Primer Mix
Instrument Part    9022147    Door Suspension Springs, QCU
Instrument Part    9020111    Tip chute, QXT
Consumable    274602    flocktype Mycoplasma Ms Ab (2)
Consumable    289991    virotype Tissue Lysis Reagent (25 ml)
Consumable    281803    virotype CSFV RT-PCR Kit (24)
Consumable    275702    flocktype Salmonella Ab (2)
Consumable    280447    virotype BTV pan/8 RT-PCR Kit (480)
Consumable    34362    TAGZyme DAPase Enzyme (2,5U)    For processing of approximay 50 mg tagged protein: 2.5 units DAPase Enzyme, 20 mM Cysteamine-HCl (1 ml)
Instrument Part    9011533    Zdrive unit, BR8000
Consumable    35370    Penta-His Alexa Fluor 647 Conjugate    125 ul Penta-His Alexa Fluor 647 Conjugate, 200 ug/ml
Consumable    959034    EZ1 RNA Tissue Mini Kit (48)    48 Reagent Cartridges (RNA Tissue) 100 Disposable Tip Holders, 100 Disposable Filter-Tips, 50 Sample Tubes (2 ml), 50 Elution Tubes (1.5 ml), Buffer RLT, RNase-Free DNase I
Sample    203601    HotStarTaq Plus DNA Polymerase (50)    Non published.
Consumable    203603    HotStarTaq Plus DNA Polymerase (250)    1 x 250 units HotStarTaq Plus DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2
Consumable    204643    QuantiTect Multiplex RT-PCR Kit (200)
Consumable    158767    Gentra Puregene Cell Kit (8 x 108)
Consumable    949020    Autopure Qubes E (192)
Consumable    203645    HotStarTaq Plus Master Mix Kit (1000)    "For 1000 x 20 μl reactions: 12 x 0.85 ml HotStarTaq Plus Master Mix, containing 1000 units of HotStarTaq Plus DNA Polymerase total, 4 x 0.55 ml CoralLoad Concentrate, 8 x 1.9 ml RNase-Free Water

HotStarTaq Plus Master Mix Kits offer all the benefits of great specificity, sensitivity, and performance as HotStarTaq Plus DNA Polymerase NOW in a master mix format, which offers even faster results and added convenience."
Consumable    201912    dNTP Set, PCR Grade 100mM, (4x100μl)    dNTP Set is a complete set of individual, highly pure dNTPs: 100 mM each dATP, dCTP, dGTP, dTTP for 1000 x 50ul PCR reactions
Consumable    12943    QIAGEN Plasmid Plus Midi Kit (25)    "25 QIAGEN Plasmid Plus Midi Columns, Extender Tubes, Reagents, Buffers, 25 QIAfilter Midi Cartridges

QIAGEN Plasmid Plus Kits enable ultrafast, large-scale plasmid purification of pure plasmid DNA. The use of a vacuum manifold allows purification of up to 12 (Mega and Giga) or up to 24 (Midi and Maxi) samples in parallel with low hands-on needs. Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. QIAGEN Plasmid Plus Kits also feature a novel wash buffer for endotoxin reduction."
Consumable    947057    BioSprint 96 One-For-All Vet Kit (384)    Kit is used on the BioSprint 96 or similar instruments in the market such as MagMAxExpress 96 from Invitrogen or KingFisher 96 from Thermo
550014    RAT MADCAM-1 PURE MAB 0.1MG OST2
550019    HU CD11B/MAC-1 APC MAB 100TST ICRF44
550024    MS CD51 PURE 0.1MG RMV-7
550025     HU P63 PURE MAB 0.1MG Y4A3
550026    MS IG ISOTYPING CBA KIT
550035    MS CD5 APC MAB 0.1MG 53-7.3
550037    HU CD51-61 PE MAB 100TST 23C6
550040    HU CD62E PE-CY5 MAB 100TST 68-5H11
550041    HU CD30 PE MAB 100TST BERH8
550042    HU CD95 PURE MAB 0.1MG EOS9.1
550052    HU CD153 (CD30L) PUR MAB 0.1MG D2-1173
550056    HAM IGG2 KPA ITCL FITC MAB 0.25MG B81-3
550057    RAT CD4 APC MAB 0.1MG OX-35
550058    MS CRRY-P65 PURE MAB 0.1MG 1F2
550059    MS CRRY-P65 BIOTIN MAB 0.1MG 1F2
550067    MS IL-4 RECOM 10UG
550068    HU GM-CSF RECOM 10UG
550069    MS IL-2 RECOM 20UG
550070    MS IL-10 RECOM 10UG
550071    HU IL-6 RECOM 10UG
550072    RAT IFN-GMA RECOM 5UG
550078    HU MIP-1 BTA PE MAB 0.1MG D21-1351
550081    MS NK-T NK CELL ATG PURE 0.5MG U5A2-13
550082    MS NK-T NK CELL ATG PE MAB 0.2MG U5A2-13
550083    MS IGG1 PE MAB 0.1MG A85-1
550084    HAM IGG2 KPA ITCL BIOTIN MAB .25MG B81-3
550085    HAM IGG2 KPA ITCL PE MAB 0.1MG B81-3
550086    MS CD120B PE MAB 0.2MG TR75-89
550252    HU CD142 PURE MAB 0.1MG HTF-1
550254    HU CD1D PURE MAB 0.1MG CD1D42
550255    HU CD1D PE MAB 100TST CD1D42
550256    HU CD73 PURE MAB 0.1MG AD2
550257    HU CD73 PE MAB 100TST AD2
550258    HU CD103 PURE MAB 0.1MG BER-ACT8
550259    HU CD103 FITC MAB 100TST BER-ACT8
550260    HU CD103 PE MAB 100TST BER-ACT8
550261    MS CD11C APC MAB 0.1MG HL3
550268    MS CD72 B-C ALOATG PURE 0.1MG JY/93
550269    MS CD72 B-C ALOATG PE 0.1MG JY/93