細胞名稱

OP9小鼠骨髓基質細胞

描述

OP9細胞株源自新生的op/op 小鼠顱蓋。因編碼M-CSF的基因中的一個突變，它不能生成有功能的巨噬細胞克隆刺激因子(M-CSF)。M-CSF的存在對胚胎干細胞(ES)分化成血細胞而不是其他巨噬細胞有抑制功能。 OP9細胞可以用于與小鼠胚胎干細胞共培養以誘導胚胎干細胞分化成成紅血球來源的、骨髓來源的和B細胞譜系的血細胞。與OP9共培養不需要外源的生長因子或復雜的胚胎結構。這個系統對研究造血細胞的發育和分化的分子機理有用。

動物種別

小鼠

性別

***

組織來源

骨髓，基質

形態

成纖維細胞

培養基和添加劑

MEMα-培養基(GIBCO,貨號12000022，添加NaHCO3 1.5g/L，肌醇 43.2mg/L, * 8.82mg/L,β-巰基乙醇 7.8mg/L)，80%；優質胎牛血清，20%。 氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度。

供應限制

REFERENCE

Nakano T , et al. Generation of lymphohematopoietic cells from embryonic stem cells in culture. Science 265: 1098-1101, 1994. PubMed: 8066449 Nakano T , et al. In vitro development of primitive and definitive erythrocytes from different precursors. Science 272: 722-724, 1996. PubMed: 8614833 Nakano T . Lymphohematopoietic development from embryonic stem cells in vitro. Semin. Immunol. 7: 197-203, 1995. PubMed: 7579206 Motoyama N , et al. bcl-x prevents apoptotic cell death of both primitive and definitive erythrocytes at the end of maturation. J. Exp. Med. 189: 1691-1698, 1999. PubMed: 10359572 Nakano T . In vitro development of hematopoietic system from mouse embryonic stem cells: a new approach for embryonic hematopoiesis. Int. J. Hematol. 65: 1-8, 1996. PubMed: 8990620 Nakano T , et al. Development of erythroid cells from mouse embryonic stem cells in culture: potential use for erythroid transcription factor study. Leukemia 3: 496-500, 1997. PubMed: 9209437 Suwabe N , et al. GATA-1 regulates growth and differentiation of definitive erythroid lineage cells during in vitro ES cell differentiation. Blood 92: 4108-4118, 1998. PubMed: 9834216 Suzuki A , Nakano T . Development of hematopoietic cells from embryonic stem cells. Int. J. Hematol. 73: 1-5, 2001. PubMed: 11372743 Eto K , et al. Megakaryocytes derived from embryonic stem cells implicate CalDAG-GEFI in integrin signaling. Proc. Natl. Acad. Sci. USA 99: 12819-12824, 2002. PubMed: 12239348