細胞名稱

MS1小鼠胰島內皮細胞

描述

MS1是1994年建株的胰島內皮細胞株。原代培養的胰島內皮細胞用抗G418的溫度敏感型SV40大T抗原(tsA-58-3)轉染。抗性克隆用克隆環分離，并篩選吸收dil-Ac-LDL的。這株細胞保留了內皮細胞的許多特性，如吸收乙 酰化LDL和表達八因子相關抗原及BEGF受體。

動物種別

小鼠

性別

***

組織來源

正常胰島內皮，SV40轉染

形態

***

培養基和添加劑

DMEM培養基(GIBCO,貨號12800017，添加NaHCO3 1.5g/L)，95%；優質胎牛血清，5%。 氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度。

供應限制

A。僅供研究之用。

REFERENCE

Arbiser JL , et al. Oncogenic H-ras stimulates tumor angiogenesis by two distinct pathways. Proc. Natl. Acad. Sci. USA 94: 861-866, 1997. PubMed: 9023347 Arbiser JL , et al. Overexpression of VEGF 121 in immortalized endothelial cells causes conversion to slowly growing angiosarcoma and high level expression of the VEGF receptors VEGFR-1 and VEGFR-2 in vivo. Am. J. Pathol. 156: 1469-1476, 2000. PubMed: 10751370 Hay, R. J., Caputo, J. L., and Macy, M. L. Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C.