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M CSF Human/ 重組人巨噬細(xì)胞集落刺激因子

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M CSF Human/ 重組人巨噬細(xì)胞集落刺激因子 產(chǎn)品編號：CYT-308
產(chǎn)品規(guī)格：2μg/10μg/1mg

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M CSF Human/ 重組人巨噬細(xì)胞集落刺激因子

產(chǎn)品編號：CYT-308
產(chǎn)品規(guī)格：2μg/10μg/1mg
 

Synonyms Macrophage Colony Stimulating Factor, CSF-1, Lanimostim, MCSF, MGC31930, M-CSF.
Introduction Granulocyte/Macrophage Colony-Stimulating Factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
Description Macrophage Colony Stimulating Factor Human Recombinant produced in E.coli is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 159 amino acids and having a total molecular mass of 36.8 KD.
MCSF is purified by proprietary chromatographic techniques.
Source Escherichia Coli.
Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder.
Formulation The protein was lyophilized with 5mM NaP, pH-8.0 & 100mM NaCl.
Solubility It is recommended to reconstitute the lyophilized M-CSF in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below
-18°C. Upon reconstitution MCSF should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.
Amino acid sequence The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Glu-Val-Ser.
Purity Greater than 97.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE.
Biological Activity The ED50, calculated by the dose-dependant stimulation of the proliferation of murine M-NFS-60 indicator cells was found to be
< 2.0ng/ml.
Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.


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