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重組小鼠白介素13/IL 13 Mouse

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重組小鼠白介素13/IL 13 Mouse 產(chǎn)品編號(hào)：CYT-375
產(chǎn)品規(guī)格：2μg/10μg/1mg

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重組小鼠白介素13/IL 13 Mouse

產(chǎn)品編號(hào)：CYT-375
產(chǎn)品規(guī)格：2μg/10μg/1mg
 

Catalogue Number CYT-375
Synonyms Interleukin-13, NC300, ALRH, BHR1, P600, IL-13, IL13.
Introduction IL13 is an immunoregulatory cytokine produced primarily by activated Th2 cells. IL-13 is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4.
Description Interleukin-13 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 110 amino acids and having a molecular mass of 12 kDa.
The IL-13 is purified by proprietary chromatographic techniques.
Source Escherichia Coli.
Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder.
Formulation The protein （1mg/ml） was lyophilized with no additives.
Solubility It is recommended to reconstitute the lyophilized Interleukin 13 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized Interleukin-13 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL13 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles.
Purity Greater than 95% as determined by SDS-PAGE.
Amino acid sequence The sequence of the first five N-terminal amino acids was determined and found to be Met-Pro-Val-Pro-Arg.
Biological Activity The ED50 range=2-6 ng/ml corresponding to a specific activity of 0.17-0.5MU/mg, determined by the dose dependent prolifiration of TF-1 cells.
Protein content Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.69 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）.

2. Analysis by RP-HPLC, using a calibrated solution of IL-13 as a Reference Standard.
Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.


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