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當前位置：賽默飛世爾科技（中國）有限公司>>細胞培養基、添加劑和試劑>>細胞培養基>>Ham's F-12 營養混合物，GlutaMAX™ 添加劑

Ham's F-12 營養混合物，GlutaMAX™ 添加劑

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產品型號Gibco

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更新時間：2023-11-06 21:22:09瀏覽次數：115次

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產品簡介

Ham's F-12 營養混合物，GlutaMAX™ 添加劑

詳細介紹

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of Gibco™ F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.


This Ham's F-12 is modified as follows:
With
• GlutaMAX™
• Phenol Red


The complete formulation is available.

Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-12 has led to a variety of improved formulations. Gibco™ F-12 with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

Gibco™ F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.


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